Whole-Cell SYBR Green I Assay for Antimalarial Activity Assessment
- Autores: Lobo E, Machado M, Murtinheira F, Nogueira F
- Ano de Publicação: 2016
- Journal: Annals of Clinical and Medical Microbiology
- Link: https://www.jscimedcentral.com/MedicalMicrobiology/medicalmicrobiology-2-1010.pdf
Malaria parasite growth assessment assays using nucleic acid dye SYBR GreenI are frequently compromised by high background readings due to haemoglobin and detergents in lysis buffer. Here, we have assessed and further validated an intact-cell SYBR Green I-based fluorescence assay (Cell-MSF) for antimalarial activity assessment even of highly fluorescent compounds.