Enhancing activity of antibiotics against Staphylococcus aureus: Zanthoxylum capense constituents and derivatives
- Autores: Cabral V, Costa SS, Couto I, Duarte A, Ferreira M-JU, Junqueira E, Luo X, Mulhovo S, Viveiros M
- Ano de Publicação: 2015
- Journal: Phytomedicine
- Link: http://www.ncbi.nlm.nih.gov/pubmed/25925969
Six compounds (1-6), isolated from the methanol extract of the roots of the African medicinal plant Zanthoxylum capense Thunb. (Rutaceae), and seven ester derivatives (7-13) were evaluated for their antibacterial activities and modulatory effects on the MIC of antibiotics (erythromycin, oxacillin, and tetracycline) and ethidium bromide (EtBr) against a Staphylococcus aureus reference strain (ATCC 6538). Using the same model, compounds 1-13 were also assessed for their potential as efflux pump inhibitors by a fluorometric assay that measures the accumulation of the broad range efflux pump substrate EtBr. Compounds 8 and 11 were further evaluated for their antibacterial, modulatory and EtBr accumulation effects against four additional S. aureus strains, which included two clinical methicillin-resistant S. aureus (MRSA) strains.