Development of a monoclonal antibody TAS-ELISA assay for detection of Phaeomoniella chlamydospora
- Autores: Cardoso F, Nascimento T, Oliveira H
- Ano de Publicação: 2014
- Journal: Phytopathologia Mediterranea
- Link: http://www.fupress.net/index.php/pm/article/view/11860
A monoclonal antibody-based triple antibody sandwich (TAS) enzyme-linked immunosorbent assay (ELISA) was developed to specifically detect Pa. chlamydospora from grapevine wood tissues. This assay could be especially useful for large-scale application in nurseries, to ensure Pa. chlamydospora-free grapevine stocks, and thus contributing to the production of healthy propagation material.
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