Gold nanoprobes for multi loci assessment of multi-drug resistant tuberculosis
- Autores: Baptista PV, Couto I, Machado D, Pedrosa P, Veigas B, Viveiros M
- Ano de Publicação: 2014
- Journal: Tuberculosis
- Link: http://www.sciencedirect.com/science/article/pii/S147297921400002X
Here we extend the Au-nanoprobe approach towards discriminating specific mutations within inhA and rpoB genes in PCR amplified DNA from isolates. Using a multiplex PCR reaction for these two genes, it is possible to assess both loci in parallel, and extend the potential of the Au-nanoprobe method to MDRTB molecular characterization with special application in the most frequent Portuguese genotypes.