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Home / Archives for Freitas FB

Freitas FB

Novel multiregion hybridization assay for the identification of the most prevalent genetic forms of the human immunodeficiency virus type 1 circulating in Portugal

  • Autores: Esteves A, Freitas FB, Parreira R, Piedade J
  • Ano de Publicação: 2013
  • Journal: AIDS Research and Human Retroviruses
  • Link: http://online.liebertpub.com/doi/abs/10.1089/aid.2012.0025

The most efficient method for HIV-1 genetic characterization involves full-genome sequencing, but the associated costs, technical features, and low throughput preclude it from being routinely used for the analysis of large numbers of viral strains. Multiregion hybridization assays (MHA) represent an alternative for a consistent genetic analysis of large numbers of viral strains. Classically, MHA rely on the amplification by real-time PCR of several regions scattered along the HIV-1 genome, and on their characterization with clade-specific TaqMan probes (also known as hydrolysis probes).
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Mosquito surveys and West Nile virus screening in two different areas of southern Portugal, 2004-2007

  • Autores: Almeida AP, Alves R, Esteves A, Freitas FB, Novo MT, Rodrigues JC, Sousa CA
  • Ano de Publicação: 2010
  • Journal: Vector-Borne and Zoonotic Diseases
  • Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Mosquito+Surveys+and+West+Nile+Virus+Screening+in+two+Different+Areas+of+Southern+Portugal

Longitudinal mosquito surveys were carried out in southern Portugal from 2004 to 2007, in a wetland area (Comporta, District of Setúbal) and around the perimeter of a dam irrigation plant that created the largest artificial lake in Europe, 250 km(2) (Alqueva, Districts of Evora and Beja).
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Species Composition and WNV Screening of Mosquitoes from Lagoons in a Wetland Area of the Algarve, Portugal.

  • Autores: de Almeida AP, Esteves A, Freitas FB, Novo MT
  • Ano de Publicação: 2011
  • Journal: Frontiers in physiology
  • Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Species+Composition+and+WNV+Screening+of+Mosquitoes+from+Lagoons+in+a+Wetland+Area+of+the+Algarve%2C+Portugal

The aim of this study was to evaluate mosquito abundance, species diversity, larval and adult population dynamics in seven lagoons integrated in the wetland coastal system of the Algarve, Portugal, in the summer of 2007, as well as the screening of these for West Nile virus (WNV).
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Molecular detection of Leishmania DNA and identification of blood meals in wild caught phlebotomine sand flies (Diptera: Psychodidae) from southern Portugal

  • Autores: Afonso MO, Campino L, Cristovao JM, Freitas FB, Maia C, Parreira R
  • Ano de Publicação: 2015
  • Journal: Parasites & Vectors
  • Link: http://www.ncbi.nlm.nih.gov/pubmed/25889732

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Satellite-derived estimation of environmental suitability for malaria vector development in Portugal

  • Autores: Almeida APG, Benali A, Freitas FB, Lima JC, Lourenço PM, Novo MT, Nunes JP, Seixas J, Sousa CA
  • Ano de Publicação: 2014
  • Journal: Remote Sensing of Environment
  • Link: http://www.sciencedirect.com/science/article/pii/S0034425714000273

This work focused on determining the current relationships between environmental factors and the density of the former malaria vector Anopheles atroparvus in Portugal, a previously endemic country. Adult females were sampled and vector density was estimated in 22 sites in Southern Portugal between 2001 and 2010 and related with land cover and satellite-derived air temperature and vegetation indices. The relationship between vector density and local larval habitat, temperature and, in a broader sense, to environmental suitability, was assessed using a statistical modelling approach.
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