Fluorimetric Methods for Analysis of Permeability, Drug Transport Kinetics, and Inhibition of the ABCB1 Membrane Transporter
- Autores: Ana Armada, António Sebastião Rodrigues, Célia Martins, Gabriella Spengler, Joseph Molnar, Leonard Amaral, Miguel Viveiros
- Ano de Publicação: 2016
- Journal: Methods in molecular biology
- Link: http://link.springer.com/protocol/10.1007%2F978-1-4939-3347-1_7
The cell membrane P-glycoprotein (P-gp; MDR1, ABCB1) is an energy-dependent efflux pump that belongs to the ATP-binding cassette (ABC) family of transporters, and has been associated with drug resistance in eukaryotic cells. Multidrug resistance (MDR) is related to an increased expression and function of the ABCB1 (P-gp) efflux pump that often causes chemotherapeutic failure in cancer.