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Home / Archives for 2016

2016

Plasmid-Borne Antimicrobial Resistance of Staphylococcus aureus Isolated in a Hospital in Lisbon, Portugal

  • Autores: Costa Sofia Santos, Couto Isabel, Fessler Andrea T., Kadlec Kristina, Melo-Cristino José, Palma Cláudia, Schwarz Stefan, Viveiros Miguel
  • Ano de Publicação: 2016
  • Journal: Microbial Drug Resistance
  • Link: http://online.liebertpub.com/doi/abs/10.1089/mdr.2015.0352

Plasmids play a key role in the genetic plasticity and survival of Staphylococcus aureus in challenging environments. Although many S. aureus plasmids have been described, still few studies portray the plasmid content of a given S. aureus population. The aim of this work was to characterize the plasmids carried by a collection of 53 S. aureus isolates collected in a large hospital in Lisbon, Portugal, and investigate their role in conferring resistance to several antimicrobial agents.
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Comparison of Leishmania typing results obtained from 16 European clinical laboratories in 2014

  • Autores: Bart A, Chicharro C, Chiodini PL, Cortes S, Davidsson L, Di Muccio T, Dujardin JC, Felger I, Grimm F, Harms G, Jaffe CL, Manser M, Paglia MG, Ravel C, Robert-Gangneux F, Roelfsema J, Töz S, Van der Auwera G, Verweij JJ
  • Ano de Publicação: 2016
  • Journal: Eurosurveillance
  • Link: http://www.eurosurveillance.org/ViewArticle.aspx?ArticleId=22662

Leishmaniasis is endemic in southern Europe, and in other European countries cases are diagnosed in travellers who have visited affected areas both within the continent and beyond. Prompt and accurate diagnosis poses a challenge in clinical practice in Europe. Different methods exist for identification of the infecting Leishmania species.
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Portugal and Angola: similarities and differences in Toxoplasma gondii seroprevalence and risk factors among pregnant women

  • Autores: de Sousa B, Lobo M L, Matos O, Patrocinio G, Sevivas T
  • Ano de Publicação: 2016
  • Journal: Epidemiology and Infection
  • Link: https://www.ncbi.nlm.nih.gov/pubmed/27608967

In this study we determined the presence of IgM/IgG antibodies to Toxoplasma gondii in sera of 155 and 300 pregnant women from Lisbon (Portugal) and Luanda (Angola), respectively, and evaluated the potential risk factors associated with this infection.
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Serological diagnosis of pneumocystosis: production of a synthetic recombinant antigen for immunodetection of Pneumocystis jirovecii

  • Ano de Publicação: 2016
  • Journal: Scientif Reports
  • Link: http://www.nature.com/articles/srep36287

This study aims to combine the production of a multi-epitope synthetic recombinant antigen (RSA) and an ELISA test for detection of anti-P. jirovecii antibodies, in order to develop a new approach for PcP diagnosis.
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ECIL guidelines for the diagnosis of Pneumocystis jirovecii pneumonia in patients with haematological malignancies and stem cell transplant recipients

  • Autores: Alanio A, Alanio A, Bretagne S, Bretagne S, Cesaro S, Cesaro S, Cordonnier C, Cordonnier C, Donnelly J P, Einsele H, Einsele H, Hauser P M, Hauser PM, Helweg-Larsen J, Helweg-Larsen J, Johan Maertens J, Lagrou K, Lagrou K, Maschmeyer G, Maschmeyer G, Matos O, Melchers W J G
  • Ano de Publicação: 2016
  • Journal: Journal of Antimicrobial Chemotherapy
  • Link: https://www.ncbi.nlm.nih.gov/pubmed/27550991

The Fifth European Conference on Infections in Leukaemia (ECIL-5) convened a meeting to establish evidence-based recommendations for using tests to diagnose Pneumocystis jirovecii pneumonia (PCP) in adult patients with haematological malignancies.
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Cercopithifilaria sp. II in Vulpes vulpes: new host affiliation for an enigmatic canine filarioid

  • Autores: André Pereira, Carla Maia, Domenico Otranto, Fábia Azevedo, Giada Annoscia, María Casero, Maria Stefania Latrofa, Vito Colella
  • Ano de Publicação: 2016
  • Journal: Parasitology Research
  • Link: http://link.springer.com/article/10.1007/s00436-016-5308-7

Cercopithifilaria bainae and Cercopithifilaria grassii (Spirurida, Onchocercidae) are filarioids inhabiting the skin of dogs worldwide. The microfilariae of a third species namely, Cercopithifilaria sp. II sensu Otranto et al. 2013, have been morphologically and molecularly characterized but scientific knowledge of this parasite is minimal.
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An Experimental Model for the Rapid Screening of Compounds with Potential Use Against Mycobacteria

  • Autores: Azzali Elisa, Coelho Tatiane, Costa Sofia Santos, Couto Isabel, da Silva Pedro Eduardo Almeida, Lopes Elizeth, Machado Diana, Pieroni Marco, Viveiros Miguel
  • Ano de Publicação: 2016
  • Journal: ASSAY and Drug Development Technologies
  • Link: http://online.liebertpub.com/doi/abs/10.1089/adt.2016.752

Infections caused by Mycobacterium tuberculosis and other mycobacteria are major challenges for global public health. Particularly worrisome are infections caused by multidrug-resistant bacteria, which are increasingly difficult to treat because of the loss of efficacy of the current antibacterial agents, a problem that continues to escalate worldwide. There has been a limited interest and investment on the development of new antibacterial agents in the past decades. This has led to the current situation, in which there is an urgent demand for innovative therapeutic alternatives to fight infections caused by multidrug-resistant pathogens, such as multidrug-resistant tuberculosis.
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Molecular epidemiology of rotavirus in four provinces of Angola before vaccine introduction

  • Autores: Dimbu R., Esteves A, Fortes F, Istrate C, Mendes C, Nordgren J, Pereira J., Saraiva N.
  • Ano de Publicação: 2016
  • Journal: Journal of Medical Virology
  • Link: http://www.ncbi.nlm.nih.gov/pubmed/26946356

Angola is a sub-Saharan country in southern Africa highly affected by diarrhoeal disease with limited epidemiological data regarding etiologic agents. This study was performed during 2012-2013, prior to rotavirus vaccine introduction, with the objective to detect and characterize the rotavirus strains circulating in four provinces of the country: Huambo, Luanda, Zaire and Cabinda.
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Prevention and control of antimicrobial resistant healthcare associated infections: the Microbiology Laboratory rocks!

  • Autores: Alexandra S. Simões, Cristina Toscano, Elsa Gonçalves, Isabel Couto, Luís V. Lapão, Miguel Viveiros, Pedro Póvoa
  • Ano de Publicação: 2016
  • Journal: Frontiers in Microbiology
  • Link: http://journal.frontiersin.org/article/10.3389/fmicb.2016.00855/full

This paper addresses the role and importance of the Microbiology Laboratory in the prevention and control of healthcare-associated infection and in Antibiotic Stewardship Programs and how it can be leveraged when combined with the use of information systems. Additionally, we critically review some challenges that the Microbiology Laboratory has to deal with, including the selection of analytic methods and the proper use of communication channels with other healthcare services.
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Etiology of Diarrhea in Children Younger Than 5 Years Attending the Bengo General Hospital in Angola

  • Autores: Brito M, Centeno-Lima S, Gasparinho C, Istrate C, Mayer AC, Mirante MC, Nery SV, Tavira L
  • Ano de Publicação: 2016
  • Journal: The Pediatric Infectious Disease Journal
  • Link: http://www.ncbi.nlm.nih.gov/pubmed/26761347

This study demonstrates high rates of infection with an enteric pathogen, particularly in children younger than 12 months, emphasizing the need to address diarrheal disease in this age group.
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