Protein Disulfide Isomerases: Impact of Thapsigargin treatment on their expression in melanoma cell lines
- Autores: Novo C, Silva Z, Veríssimo T, Videira PA
- Ano de Publicação: 2015
- Journal: IJBIOMAC
- Link: http://www.ncbi.nlm.nih.gov/pubmed/25912252
Anti-cancer treatments usually elevate the content of unfolded or misfolded proteins in the endoplasmic reticulum (ER). Here we aimed to get insights into the relation between sensitivity of melanoma cell lines to the ER stress inducer thapsigargin (THG) and the genetic expression of protein disulfide isomerase family members (PDIs). The expression of PDIs was analysed by flow cytometry and real-time PCR.