Differences between synthetic ß-haematin and native hemozoin crystals
- Autores: Carvalho P.A., F. Nogueira, L. Coelho, R.C. Martins
- Ano de Publicação: 2013
- Journal: Microscopy and Microanalysis
Instituto de Higiene e Medicina Tropical
Chikungunya virus (CHIKV) is a mosquito-transmitted alphavirus responsible for painful disablingpolyarthritis, undergoing re-emergence in the Indian Ocean and the Asian continent. Travelling and changing patterns of vector distribution, and abundance due to climate changes, make CHIKV a global threat without effective control strategies. One approach to reduce the CHIKV burden is the development of a vaccine. Virus-like particles (VLPs) are a safe and highly effective class of subunit vaccines that mimic the overall structure of virus particles. Accordingly, we aim to produce CHIKV VLPs in a baculovirus-insect cell system, well known for allowing high yields of recombinant protein expression, to be used as a vaccine preparation.
This work reports the initial characterization, including near full-length sequence analysis, of an ISF which seems to form a divergent lineage within the ISF radiation, as indicated by phylogenetic analysis based on ac multiple sequence alignment of the NS5 flavivirus coding sequence.
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