Identification of efflux pump-mediated multidrug-resistant bacteria by the ethidium bromide-agar cartwheel method.

• Autores: Amaral L, Costa SS, Couto I, Fanning S, Martins M, Pacheco T, Pagès JM, Viveiros M
• Ano de Publicação: 2011
• Journal: In vivo
• Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Identification+of+efflux+pump+mediated+multi-drug+resistant+bacteria+by+the+ethidium+bromide-agar+cartwheel+method

BACKGROUND/AIM:
Bacterial multidrug resistance may be mediated by the overexpression of efflux pumps. Conventional evaluation of efflux activity using efflux pump substrates, such as ethidium bromide, requires specialised instrumentation. The agar-based method, previously reported, has been modified to evaluate as many as twelve bacterial strains and has been termed the ethidium bromide-agar cartwheel method.
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