Identification of efflux pump-mediated multidrug-resistant bacteria by the ethidium bromide-agar cartwheel method.
- Autores: Amaral L, Costa SS, Couto I, Fanning S, Martins M, Pacheco T, Pagès JM, Viveiros M
- Ano de Publicação: 2011
- Journal: In vivo
- Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Identification+of+efflux+pump+mediated+multi-drug+resistant+bacteria+by+the+ethidium+bromide-agar+cartwheel+method
Bacterial multidrug resistance may be mediated by the overexpression of efflux pumps. Conventional evaluation of efflux activity using efflux pump substrates, such as ethidium bromide, requires specialised instrumentation. The agar-based method, previously reported, has been modified to evaluate as many as twelve bacterial strains and has been termed the ethidium bromide-agar cartwheel method.