Multilocus sequence typing (MLST) for lineage assignment and high resolution diversity studies in Trypanosoma cruzi.
- Autores: Acosta N, Bhattacharyya T, Carrasco HJ, Diosque P, Lewis MD, Llewellyn MS, Mauricio IL, Messenger LA, Miles MA, Yeo M
- Ano de Publicação: 2011
- Journal: Plos Neglected Tropical Diseases
- Link: http://www.ncbi.nlm.nih.gov/pubmed/?term=Multilocus+sequence+typing+(MLST)+for+lineage+assignment+and+high+resolution+diversity+studies+in+Trypanosoma+cruzi
Multilocus sequence typing (MLST) is a powerful and highly discriminatory method for analysing pathogen population structure and epidemiology. Trypanosoma cruzi, the protozoan agent of American trypanosomiasis (Chagas disease), has remarkable genetic and ecological diversity. A standardised MLST protocol that is suitable for assignment of T. cruzi isolates to genetic lineage and for higher resolution diversity studies has not been developed.