Novel multiregion hybridization assay for the identification of the most prevalent genetic forms of the human immunodeficiency virus type 1 circulating in Portugal
- Autores: Esteves A, Freitas FB, Parreira R, Piedade J
- Ano de Publicação: 2013
- Journal: AIDS Research and Human Retroviruses
- Link: http://online.liebertpub.com/doi/abs/10.1089/aid.2012.0025
The most efficient method for HIV-1 genetic characterization involves full-genome sequencing, but the associated costs, technical features, and low throughput preclude it from being routinely used for the analysis of large numbers of viral strains. Multiregion hybridization assays (MHA) represent an alternative for a consistent genetic analysis of large numbers of viral strains. Classically, MHA rely on the amplification by real-time PCR of several regions scattered along the HIV-1 genome, and on their characterization with clade-specific TaqMan probes (also known as hydrolysis probes).